References [ 5 ]
Costas M & Griffiths AJ (1984) The esterases and acid-phosphatases of Acanthamoeba (Amoebida, Acanthamoebidae). Protistologica 20(1): 33-41.
DOI: none
Daggett PM, Sawyer T & Nerad T (1982) Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microbial Ecology 8(4): 371-386.
DOI: 10.1007/BF02010676
Kong, HH, Shin JY, Yu HS, Kim J, Hahn TW, Hahn YH & Chung DI (2002) Mitochondrial DNA restriction fragment length polymorphism (RFLP) and 18S small-subunit ribosomal DNA PCR-RFLP analyses of Acanthamoeba isolated from contact lens storage cases of residents in southwestern Korea Journal of Clinical Microbiology 40: 1199-1206.
Reveiller FL, Marciano-Cabral F, Pernin P, Cabanes PA & Legastelois S (2000) Species specificity of a monoclonal antibody produced to Naegleria fowleri and partial characterisation of its antigenic determinant. Parasitology Research 86: 634-641.
DOI: none
Martinez AJ, Markowitz SM & Duma RJ (1975) Experimental pneumonitis and encephalitis caused by Acanthamoeba in mice: pathogenesis and ultrastructural features. The Journal of Infectious Diseases 131(6): 692-699.
Division/Phylum: Amoebozoa Class: Discosea
Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology
and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee
that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive
from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and
Adl et al. (2019) for protists.
Culture media, purity and growth conditions:
Medium:
NN;
Monoxenic; maintained by serial subculture and cryopreserved; contains non-pathogenic E. coli as food source.
| Attributes | |
| Authority | Sawyer, Visvesvera & Harke 1977 |
| Isolator | Chang (1959) |
| Collection Site | private well water nr. Cincinnati, Ohio, USA |
| Notes | Originally identified by isolator as Hartmanella glebae, deposited as A. castellanii, re-identified as A. hatchetti in 1994 based on RFLP analysis of mitochondrial DNA |
| Axenicity Status | Monoxenic |
| Area | North America |
| Country | USA |
| Environment | Freshwater |
| GMO | No |
| Group | Protozoa |
| In Scope of Nagoya Protocol | No |
| ABS Note | Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain. |
| Collection Date | 1959 |
| Pathogen | Potential Pathogen: Hazard Class 2 |
| Strain Maintenance Sheet | SM_Acanthamoeba.pdf |
| Toxin Producer | Not Toxic / No Data |
| Type Culture | No |
| Taxonomy WoRMS ID | |
| Equivalent Strains | ATCC 30898 |
| Formerly Listed in CCAP as | Acanthamoeba castellanii (Douglas) Volkonsky 1931 |
CCAP 1501/2B
Acanthamoeba hatchetti
- Product Code: CCAP 1501/2B
- Availability: Potential Pathogen
You are ordering Pathogenic strains - we cannot supply these via online ordering. Please contact CCAP directly. You will be required to download and submit Form A before any pathogenic strains are despatched.