References [ 13 ]
Costas M & Griffiths AJ (1984) The esterases and acid-phosphatases of Acanthamoeba (Amoebida, Acanthamoebidae). Protistologica 20(1): 33-41.
DOI: none
Walochnik J, Aichelburg A, Assadian O, Steuer A, Visvesvara G, Vetter N & Aspöck H (2008) Granulomatous amoebic encephalitis caused by Acanthamoeba amoebae of genotype T2 in a Human Immunodeficiency Virus-negative patient. Journal of Clinical Microbiology 46: 338-340.
DOI: 10.1128/JCM.01177-07
Garate M, Marchant J, Cubillos I, Cao Z, Khan NA & Panjwani N (2006) In vitro pathogenicity of Acanthamoeba is associated with the expression of the mannose-binding protein. Investigative Ophthalmology and Visual Science 47: 1056-1062.
DOI: 10.1167/iovs.05-0477
Maghsood AH, Sissons J, Rezaian M, Nolder D, Warhurst D & Khan NA (2005) Acanthamoeba genotype T4 from the UK and Iran and isolation of the T2 genotype from clinical isolates. Journal of Medical Microbiology 54: 755-759.
Lorenzo-Morales J, Ortega-Rivas A, Foronda P, Abreu-Acosta N, Ballart D, Martínez E & Valladares B (2005) RNA interference (RNAi) for the silencing of extracellular serine proteases genes in Acanthamoeba: Molecular analysis and effect on pathogenicity. Molecular and Biochemical Parasitology 144: 10-15.
Stothard DR, Schroeder-Diedrich JM, Awwad MH, Gast RJ, Ledee DR, Rodriguez-Zaragoza S, Dean CL, Fuerst P & Byers TJ (1998) The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. Journal of Eukaryotic Microbiology 45: 45-54.
DOI: none
Stothard DR, Hay J, Schroeder-Diedrich JM, Seal DV & Byers TJ (1999) Fluorescent oligonucleotide probes for clinical and environmental detection of Acanthamoeba and the T4 18S rRNA gene sequence type. Journal of Clinical Microbiology 37: 2687-2693.
DOI: none
Khan NA, Jarroll EL, Panjwani N, Cao Z & Paget TA (2000) Proteases as markers for differentiation of pathogenic and nonpathogenic species of Acanthamoeba. Journal of Clinical Microbiology 38: 2858-2861.
DOI: none
Weekers PHH, Bodelier PLE, Wijen JPH & Vogels GD (1993) Effects of grazing by the free-living soil amoebae Acanthamoeba castellanii, Acanthamoeba polyphaga, and Hartmannella vermiformis on various bacteria. Applied and Environmental Microbiology 59: 2317-2319.
DOI: none
Fuerst PA, Booton GC & Crary M (2014) Phylogenetic analysis and the evolution of the 18S rRNA gene typing system of Acanthamoeba. Journal of Eukaryotic Microbiology 62: 69-84.
DOI: 10.1111/jeu.12186
Fuerst, PA (2023) The status of molecular analyses of isolates of Acanthamoeba maintained by international culture collections. Microorganisms 11(2): 295.
Fuerst PA & Booton GC (2020) Species, sequence types and alleles: Dissecting genetic variation in Acanthamoeba Pathogens 9(7): 534.
Corsaro D, Walochnik J, Köhsler M & Rott MB (2015) Acanthamoeba misidentification and multiple labels: redefining genotypes T16, T19, and T20 and proposal for Acanthamoeba micheli sp. nov. (genotype T19) Parasitology Research 114(7): -.
Division/Phylum: Amoebozoa Class: Discosea
Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology
and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee
that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive
from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and
Adl et al. (2019) for protists.
Culture media, purity and growth conditions:
Medium:
NN;
Monoxenic; maintained by serial subculture and cryopreserved; non-pathogenic E. coli food source.
| Attributes | |
| Authority | (Reich) Page 1967 |
| Isolator | Sawyer (1967) |
| Collection Site | old distilled water carboy USA |
| Notes | isolated by plating; clonal culture; isolated at Oxford Biological Laboratory, US Dept of Commerce, Oxford, MD 21654, USA - possibly originated there. |
| Axenicity Status | Monoxenic |
| Area | North America |
| Country | USA |
| Environment | Freshwater |
| GMO | No |
| Group | Protozoa |
| In Scope of Nagoya Protocol | No |
| ABS Note | Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain. |
| Collection Date | 1967 |
| Original Designation | OX-1 |
| Pathogen | Potential Pathogen: Hazard Class 2 |
| Strain Maintenance Sheet | SM_Acanthamoeba.pdf |
| Toxin Producer | Not Toxic / No Data |
| Type Culture | No |
| Taxonomy WoRMS ID | 956806 |
| Formerly Listed in CCAP as | Acanthamoeba polyphaga (Puschkarew) Volkonsky 1931 |
CCAP 1501/3C
Acanthamoeba palestinensis
- Product Code: CCAP 1501/3C
- Availability: Potential Pathogen
You are ordering Pathogenic strains - we cannot supply these via online ordering. Please contact CCAP directly. You will be required to download and submit Form A before any pathogenic strains are despatched.