Division/Phylum: Discoba Class: Heterolobosea Order: Schizopyrenida
Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology
and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee
that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive
from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and
Adl et al. (2019) for protists.
Culture media, purity and growth conditions:
Medium:
wMY agar;
Bacteria present; maintained by serial subculture and cryopreserved; 15-25 deg C; ambient lighting; subculture 1-2 weeks
| Attributes | |
| Isolator | Becker & Silberman (2015) |
| Collection Site | Faecal sample taken from a female Ringed Salamander (Ambystoma annulatum) Small pond in Tontitown, Arkansas |
| Archive Date | 2024-04-10 12:19:24 |
| Archive Reason | Temporary - New Strain |
| Notes | Isolation: a faecal sample was aseptically coaxed from a gravid adult female A. annulatum, where she directly deposited it onto a weak malt-yeast agar plate (wMY) in the middle of a uniform lawn of E. coli; Type specimen of a new species of Naegleria isolated from the gut contents of a salamander "of special concern" that has a very small population size and is endemic to the Ozark Mountains of the central USA. This amoeba is likely amphizoic and is the first gut-inhabiting protist described from a salamander host. Additionally, it is likely an early branching representative within the genus Naegleria. To date only amoebae and cyst stages have been documented. Typical heterolobosean morphology - trophic amoebae and cyst cells possess a single nucleus with a large central nucleolus. Amoebae move with eruptive pseudopodia. A bulbus uroid was occasionally observed as were trailing cytoplasmic filaments. |
| Axenicity Status | Bacteria present |
| Area | North America |
| Country | USA |
| Environment | Animal |
| GMO | No |
| Group | Protozoa |
| Latitude | 36 09' 28.50" N |
| Longitude | 94 16' 00.82" W |
| In Scope of Nagoya Protocol | No |
| ABS Note | Collected post Nagoya Protocol from a country with no ABS measures at time of access. No known Nagoya Protocol restrictions for this strain. |
| Collection Date | November 2015 |
| Pathogen | Not pathogenic: Hazard Class 1 |
| Toxin Producer | Not Toxic / No Data |
| Type Culture | Yes |
CCAP 1518/36
Naegleria lustrarae
- Product Code: CCAP 1518/36
- Availability: Archived
This strain is Archived, see strain data below for archive reason. Please contact us for more information.