• Flamella piscinae
References [ 1 ]
Walthall AC, Tice AK & Brown MW (2016) A New Species of Flamella (Amoebozoa, Variosea, Gracilipodida) Isolated from a Freshwater Pool in Southern Mississippi, USA Acta Protozoologica 55: 111-117.
Division/Phylum: Amoebozoa Class: Variosea

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: NN; Monoxenic; maintained by serial subculture; Contains E. coli food source. Room temp, ambient lighting, pH ~7.4. Will cryopreserve.
Attributes
AuthorityBrown & Walthall 2016
IsolatorBrown (2015)
Collection Sitetop of impermeable swimming pool cover, 4cm deep, lots of algae on cover Petal, Mississippi, USA
Notes Isolation: water sample plated onto sterile wMY agar petri dish and incubated. Water sample collected in winter from top of pool cover; Freshwater inhabiting fan-shaped amoeba; Length in locomotion 14-23 µm, breadth 18-39 µm. Cells with one or two nuclei. Cytsformer. Clonal culture. Image supplied by Depositor: A - locomotive form with trailing filaments; B - locomotive form; C - locomotive form with subpseudopodia; D - locomotive form; E - cytokinesis; F - floating form; G - floating form; H - single cyst; I - cyst enveloped within multiple walls; J - cysts sharing walls; K - cysts sharing walls in a linear conformation. Scale bar: 10 µm. All images are to scale.
Axenicity Status Monoxenic
Area North America
Country USA
Environment Freshwater
GMO No
Group Protozoa
Latitude 31.339423N
Longitude -89.194171 W
In Scope of Nagoya Protocol No
ABS Note Collected post Nagoya Protocol from a country with no ABS measures at time of access. No known Nagoya Protocol restrictions for this strain.
Collection Date 01/03/2015
Original Designation GFP151sc
Pathogen Not pathogenic: Hazard Class 1
Strain Maintenance Sheet
Toxin Producer Not Toxic / No Data
Type Culture Yes
Taxonomy WoRMS ID

CCAP 1525/5

Flamella piscinae