• Nuclearia thermophila
References [ 3 ]
Dirren S, Salcher MM, Blom JF, Schweikert M & Posch T (2014) Menage-a-trois: The amoeba Nuclearia sp. from Lake Zurich with its ecto- and endosymbiotic bacteria. Protist 165: 745-758.
Dirren S & Posch T (2016) Promiscuous and specific bacterial symbiont acquisition in the amoeboid genus Nuclearia (Opisthokonta FEMS Microbiology Ecology 92: fiw105.
Dirren S, Pitsch G, Silva MOD & Posch T (2017) Grazing of Nuclearia thermophila and Nuclearia delicatula (Nucleariidae, Opisthokonta) on the toxic cyanobacterium Planktothrix rubescens European Journal of Protistology 60: 87-101.
Sequences [ 1 ]
EMBL/Genbank Links
(Bold text = submission by CCAP staff or collaborators)
Division/Phylum: Opisthokonta Class: Nucleariidae/Fonticula gp

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: MW; Bacteria present; Cryopreserved; with Planktothrix rubescens as food source; 18 deg C; pH 8.1; 12h:12h cycle 5-15µmol m-2 s-1
Attributes
AuthorityYoshida, Nakayama & Inouye 2009
IsolatorDirren (2011)
Collection Site Lake Zurich, Switzerland
Notes Isolation: picking and washing of individual cells with a micropipette; Food source is CCAP 1459/40 Plantothrix rubescens, this is a toxic strain.
Axenicity Status Bacteria present
Area Europe
Country Switzerland
Environment Freshwater
GMO No
Group Protozoa
Latitude 47 19' 11.5" N
Longitude 8 33' 10.1" E
In Scope of Nagoya Protocol No
ABS Note Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain.
Collection Date c 2011
Original Designation SD2011 / Strain N
Pathogen Not pathogenic: Hazard Class 1
Strain Maintenance Sheet SM_Nuclearia.pdf
Toxin Producer Not Toxic / No Data
Type Culture No
Taxonomy WoRMS ID 707589
Formerly Listed in CCAP asNuclearia sp.

CCAP 1552/5

Nuclearia thermophila