References [ 1 ]
van Ooijen G, Knox K, Kis K, Bouget FY & Millar AJ (2012) Genomic transformation of the picoeukaryote Ostreococcus tauri. Journal of Visualized Experiments (65): e4074.
Division/Phylum: Chlorophyta Class: Prasinophyceae Order: Mamiellales

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: K; Bacteria present; Cryopreserved; 30ppt salinity; Keller Medium or f/2; 20 deg C; pH 8.3; 12h:12h light:dark
Attributes
AuthorityCourties & Chrétiennot-Dinet 1995
IsolatorCourties & Chrétiennot-Dinet (1995)
Collection Site Wild Type collected from Thau Lagoon, Mediterranean
Archive Date 2019-10-15 14:37:37
Archive Reason Temporary - Poor/Contaminated
Notes Transgenic strain - made from the LHY line expressing GFP (unpublished). It is necessary to grow in Keller medium in order to get expression of the transgene, due to the difference in inorganic and organic phosphate between the two media. Wild Type (CCAP 157/2 Ostreococcus tauri OTTH 0595) collected from lagoon with salinity ranging from 24ppt to 38ppt. Grows better in glass with blue light, i.e. blue cellophane over the light source; Sub interval 2 weeks.
Axenicity Status Bacteria present
Environment Marine
In Scope of Nagoya Protocol No
Collection Date pre 2014
Original Designation ro GFP cyt #3
Pathogen Not pathogenic: Hazard Class 1
Toxin Producer Not Toxic / No Data
Type Culture No

CCAP 157/4

Ostreococcus tauri - ro GFP3

  • Product Code: CCAP 157/4
  • Availability: Archived
This strain is Archived, see strain data below for archive reason. Please contact us for more information.