References [ 4 ]
Qvarnstrom Y, da Silva AJ, Schuster FL, Gelman BB & Visvesvara GS (2009) Molecular confirmation of Sappinia pedata as a causative agent of amoebic encephalitis The Journal of Infectious Diseases 199: 1139-1142.
DOI: 10.1086/597473
Tyml T & Dyková I (2018) Sappinia sp. (Amoebozoa: Thecamoebida) and Rosculus sp. (SAR: Cercozoa) Isolated From King Penguin Guano Collected in the Subantarctic (South Georgia, SalisburyPlain) and their Coexistence in Culture Journal of Eukaryotic Microbiology -: -.
DOI: 10.1111/jeu.12500
Wylezich C, Walochnik J & Michel R (2009) High genetic diversity of Sappinia-like strains (Amoebozoa, Thecamoebidae) revealed by SSU rRNA investigations. Parasitology Research 105: 869-873.
Walochnik J, Wylezich C & Michel R (2010) the genus Sappinia: History, phylogeny and medical relevance. Experimental Parasitology 126(1): 4-13.
Division/Phylum: Amoebozoa Class: Discosea
Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology
and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee
that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive
from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and
Adl et al. (2019) for protists.
Culture media, purity and growth conditions:
Medium:
NN;
Bacteria present; maintained by serial subculture; 20 deg C, no lighting; E. coli as food bacteria
| Attributes | |
| Authority | Hartmann & Nägler 1908 |
| Isolator | Michel (2002) |
| Collection Site | bark of a plane tree by Rhine River, Andernach, Germany |
| Notes | Isolation: a piece of bark was transferred to NN agar plate; originally grown on NN with 1ppt seasalt; Lost in 2006, resupplied by depositor March 07; Sappinia diploidea is possibly pathogenic and as such requires an authorisation form completing before we can supply. See Walochnik, Wylezich & Michel 2010 and Visvesvara, Moura & Schuster, 2007. |
| Axenicity Status | Bacteria present |
| Area | Europe |
| Country | Germany |
| Environment | Plant |
| GMO | No |
| Group | Protozoa |
| In Scope of Nagoya Protocol | No |
| ABS Note | Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain. |
| Collection Date | c 2002 |
| Original Designation | Sd-plat-4/02 |
| Pathogen | Potential Pathogen: Hazard Class 2 |
| Strain Maintenance Sheet | |
| Toxin Producer | Not Toxic / No Data |
| Type Culture | No |
| Taxonomy WoRMS ID | |
CCAP 1575/2
Sappinia diploidea
- Product Code: CCAP 1575/2
- Availability: Potential Pathogen
You are ordering Pathogenic strains - we cannot supply these via online ordering. Please contact CCAP directly. You will be required to download and submit Form A before any pathogenic strains are despatched.