• Thecamoeba quadrilineata
References [ 5 ]
Michel R, Müller K-D & Hauröder B (2009) A novel microsporidian endoparasite replicating within the nucleus of Saccamoeba limax isolated from a pond. Endocytobiosis Cell Research 19: 120-126.
DOI: none
Michel R, Walochnik J & Scheid P (2014) Article for the "Free-living amoebae Special Issue": Isolation and characterisation of various amoebophagous fungi and evaluation of their prey spectrum. Experimental Parasitology 145: S131-S136.
Koehsler M, Michel R, Wylezich C, Lugauer J & Walochnik J (2007) Molecular identification and classification of Cochlonema euryblastum, a zoopagalean parasite of Thecamoeba quadrilineata. Mycologia 99: 215-221.
DOI: none
Mesentsev YS & Smirnov AV (2019) Thecamoeba cosmophorea n. sp. (Amoebozoa, Discosea, Thecamoebida) - An example of sibling species within the genus Thecamoeba European Journal of Protistology 67: 132-141.
Michel R & Wylezich C (2005) Beitrag zur Biologie und Morphologie von Cochlonema euryblastum, einem endo-parasitischen Pilz von Thecamoeba quadrilineata Mikrokosmos 94(2): 75-79.
DOI: none
Division/Phylum: Amoebozoa Class: Discosea

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: NPA; Bacteria present; maintained by serial subculture; 20 deg C, no lighting; E. coli as food source
Attributes
AuthorityCarter 1856
IsolatorMichel (1998)
Collection Siteroof gutter sediment Melsbach, Germany
Notes Isolation: various samples of the sediment were transferred to NN agar plates; Before deposit this Thecamoeba was found to be infected by a large fungus-like parasite, Cochlonema euryblastum (CCAP 3010/1), see Michel & Wylezich 2005; Strain lost in 2006, resupplied by depositor March 07
Axenicity Status Bacteria present
Area Europe
Country Germany
Environment Freshwater
GMO No
Group Protozoa
In Scope of Nagoya Protocol No
ABS Note Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain.
Collection Date c 1998
Original Designation Dch-1
Pathogen Not pathogenic: Hazard Class 1
Strain Maintenance Sheet
Toxin Producer Not Toxic / No Data
Type Culture No
Taxonomy WoRMS ID 616591

CCAP 1583/10

Thecamoeba quadrilineata