Division/Phylum: Amoebozoa Class: Discosea
Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology
and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee
that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive
from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and
Adl et al. (2019) for protists.
Culture media, purity and growth conditions:
Medium:
0.025% Cerophyl infusion in PJ medium (see notes below);
Bacteria and other organisms present; maintained by serial subculture; 18-20 deg C; no lighting required
| Attributes | |
| Authority | Ehrenberg 1838 |
| Isolator | Kudryavtsev (2006) |
| Collection Site | mud sediments, filter system of a private freshwater aquarium Berlin, Germany |
| Notes | Isolation: enrichment cultivation with subsequent purification by pipetting; contains Paravannella minima (a very tiny vannellid amoeba that serves also as food source); medium: 250mg Cerophyl boiled for 5 minutes in 1 litre of PJ, filtered and autoclaved; the culture is relatively stable, but does not reach especially high denisities, a few of the cells can survive in the petri dishes up to 2.5-3 months if the medium does not dry out; amoeba is relatively large (up to 100 microns during locomotion), elongated during locomotion with pointed posterior end. The locomotive morphotype is that of a rugose Thecamoeba with wrinkled surface. |
| Axenicity Status | Bacteria and other organisms present |
| Area | Europe |
| Country | Germany |
| Environment | Freshwater |
| GMO | No |
| Group | Protozoa |
| In Scope of Nagoya Protocol | No |
| ABS Note | Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain. |
| Collection Date | c 2006 |
| Pathogen | Not pathogenic: Hazard Class 1 |
| Strain Maintenance Sheet | |
| Toxin Producer | Not Toxic / No Data |
| Type Culture | Yes |
| Taxonomy WoRMS ID | |
CCAP 1583/13
Thecamoeba verrucosa
- Product Code: CCAP 1583/13
- Availability: See Availability/Lead Times