• Vacuolamoeba acanthoformis
References [ 1 ]
Tice AK, Shadwick LL, Fiore-Donno AM, Geisen S, Kang S, Schuler GA, Spiegel FW, Wilkinson KA, Bonkowski M, Dumack K, Lahr DJG, Voelcker E, Clauß S, Zhang J & Brown MW (2016) Expansion of the molecular and morphological diversity of Acanthamoebidae (Centramoebida, Amoebozoa) and identification of a novel life cycle type within the group. Biology Direct 11: 69.
Sequences [ 1 ]
EMBL/Genbank Links
(Bold text = submission by CCAP staff or collaborators)
Division/Phylum: Amoebozoa Class: Discosea

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: NN; Bacteria present; maintained by serial subculture; E.coli food source; 22-25 deg C; 3-4 weeks sub interval
Attributes
AuthorityTice, Geisen & Brown 2016
IsolatorGeisen (2014)
Collection Sitemeadow, altitude >4,100m Mila Mountain, Maizhokunggar County, Lhasa, Tibet
Climatic Zone Mountain
Notes Isolation: single cell; cystformer; mean cell length 22.5 µm, irregular shaped amoebae, acanthopodial extensions can form from all areas of the cell body. Cells most often with 1 vesicular nucleus with a central nucleolus. Cells with 2 nuclei have been observed, cell body often has 4-5 vacuoles, sometimes with one or more contractile vacuoles, cysts round to irregularly shaped with a single wall; image key - P,Q&S) trophic cell R) cyst
Axenicity Status Bacteria present
Area Asia
Country Tibet
Environment Soil
GMO No
Group Protozoa
Latitude 29.7 N
Longitude 92.166667 E
In Scope of Nagoya Protocol No
ABS Note Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain.
Collection Date pre June 2014
Original Designation Tib 84
Pathogen Not pathogenic: Hazard Class 1
Strain Maintenance Sheet
Toxin Producer Not Toxic / No Data
Type Culture Yes
Taxonomy WoRMS ID

CCAP 2580/1

Vacuolamoeba acanthoformis