References [ 16 ]

Kilvington S & Beeching J (1995) Identification and epidemiological typing of Naegleria fowleri with DNA probes. Applied and Environmental Microbiology 61(6): 2071-2078.

DOI: none

Kilvington S & Beeching J (1995) Development of a PCR for identification of Naegleria fowleri from the environment. Applied and Environmental Microbiology 61(10): 3764-3767.

DOI: none

Kilvington S, Gray T, Dart J, Morlet N, Beeching JR, Frazer DG & Matheson M (2004) Acanthamoeba keratitis: The role of domestic tap water contamination in the United Kingdom. Investigative Ophthalmology and Visual Science 45: 165-169.

DOI: 10.1167/iovs.03-0559

Pickup ZL, Pickup R & Parry JD (2007) Effects of bacterial prey species and their concentration on growth of the amoebae Acanthamoeba castellanii and Hartmannella vermiformis. Applied and Environmental Microbiology 73(8): 2631-2634.

DOI: 10.1128/AEM.02061-06

Taylor SJ, Ahonen LJ, de Leij FAAM & Dale JW (2003) Infection of Acanthamoeba castellanii with Mycobacterium bovis and M. bovis BCG and survival of M. bovis within the amoebae. Applied and Environmental Microbiology 69(7): 4316-4319.

DOI: 10.1128/AEM.69.7.4316-4319.2003

Costas M & Griffiths AJ (1984) The esterases and acid-phosphatases of Acanthamoeba (Amoebida, Acanthamoebidae). Protistologica 20(1): 33-41.

DOI: none

Costas M & Griffiths AJ (1984) Taxonomic significance of the fatty acid composition of Acanthamoeba (Amoebida, Acanthamoebidae). Protistologica 20(1): 27-31.

DOI: none

Tsvetkova N, Schild M, Panaiotov S, Kurdova-Mintcheva R, Gottstein B, Walochnik J, Aspöck H, Lucas MS & Müller N (2004) The identification of free-living environmental isolates of amoebae from Bulgaria. Parasitology Research 92: 405-413.

DOI: 10.1007/s00436-003-1052-x

Beattie TK & Tomlinson A (2009) The effect of surface treatment of silicone hydrogel contact lenses on the attachment of Acanthamoeba castellanii trophozoites. Eye & Contact Lens: Science & Clinical Practice 35(6): 316-319.

DOI: 10.1097/ICL.0b013e3181becce6

Wright SJL, Redhead K & Maudsley H (1981) Acanthamoeba castellanii, a predator of cyanobacteria. Journal of General Microbiology 125: 293-300.

DOI: 10.1099/00221287-125-2-293

Mardare C, Delahay RJ & Dale JW (2013) Environmental amoebae do not support the long-term survival of virulent mycobacteria. Journal of Applied Phycology 114: 1388-1394.

DOI: 10.1111/jam.12166

Kilvington S, Beeching JR & White DG (1991) Differentiation of Acanthamoeba strains from infected corneas and the environment by using restriction endonuclease digestion of whole-cell DNA. Journal of Clinical Microbiology 29: 310-314.

DOI: none

Hughes R, Andrew PW & Kilvington S (2003) Enhanced killing of Acanthamoeba cysts with a plant peroxidase-hydrogen peroxide-halide antimicrobial system. Applied and Environmental Microbiology 69: 2563-2567.

DOI: 10.1128/AEM.69.5.2563-2567.2003

Martín-Pérez T, Criado-Fornelio A, Avila-Blanco M & Pérez-Serrano J (2018) Development and optimization of new culture media for Acanthamoeba spp. (Protozoa: Amoebozoa) European Journal of Protistology 64: 91-102.

DOI: 10.1016/j.ejop.2018.04.002

Fuerst, PA (2023) The status of molecular analyses of isolates of Acanthamoeba maintained by international culture collections. Microorganisms 11(2): 295.

DOI: 10.3390/microorganisms11020295

Corsaro D, Mrva M, Colson P & Walochnik J (2024) Validation and redescription of Acanthamoeba terricola Pussard, 1964 (Amoebozoa: Acanthamoebidae) European Journal of Protistology 94: 126091.

DOI: 10.1016/j.ejop.2024.126091

Division/Phylum: Amoebozoa Class: Discosea

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:

Medium: NN; Monoxenic; maintained by serial subculture and cryopreserved; contains non-pathogenic E. coli as food source.

Attributes
Authority	(Douglas) Volkonsky 1931
Isolator	Neff (1957)
Collection Site	Pacific Grove, California
Notes	This strain may be Acanthamoeba terricola Pussard 1964, according to Corsaro et al. 2024, our sequence (although short) is a 100% match to both species, for now we are retaining the original strain name (Nov24)
Axenicity Status	Monoxenic
Area	North America
Country	USA
Environment	Soil
GMO	No
Group	Protozoa
In Scope of Nagoya Protocol	No
ABS Note	Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain.
Collection Date	1957
Pathogen	Potential Pathogen: Hazard Class 2
Strain Maintenance Sheet	SM_Acanthamoeba.pdf
Toxin Producer	Not Toxic / No Data
Type Culture	No
Taxonomy WoRMS ID

CCAP 1501/1A

Acanthamoeba castellanii "Neff Strain"

Product Code: CCAP 1501/1A
Availability: Potential Pathogen

You are ordering Pathogenic strains - we cannot supply these via online ordering. Please contact CCAP directly. You will be required to download and submit Form A before any pathogenic strains are despatched.

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