References [ 7 ]
Costas M & Griffiths AJ (1984) The esterases and acid-phosphatases of Acanthamoeba (Amoebida, Acanthamoebidae). Protistologica 20(1): 33-41.
DOI: none
Costas M & Griffiths AJ (1984) Taxonomic significance of the fatty acid composition of Acanthamoeba (Amoebida, Acanthamoebidae). Protistologica 20(1): 27-31.
DOI: none
Whan L, Grant IR & Rowe MT (2006) Interaction between Mycobacterium avium subsp. paratuberculosis and environmental protozoa. BMC Microbiology 6: 63.
Weekers PHH, Bodelier PLE, Wijen JPH & Vogels GD (1993) Effects of grazing by the free-living soil amoebae Acanthamoeba castellanii, Acanthamoeba polyphaga, and Hartmannella vermiformis on various bacteria. Applied and Environmental Microbiology 59: 2317-2319.
DOI: none
De Obeso Fernandez Del Valle A, Lorenzo-Morales J & Maciver SK (2017) Leptomyxa valladaresi n. sp. (Amoebozoa, Tubulinea, Leptomyxida), from Mount Teide, Tenerife, Spain. Experimental Parasitology 183: 85-91.
Fuerst, PA (2023) The status of molecular analyses of isolates of Acanthamoeba maintained by international culture collections. Microorganisms 11(2): 295.
Corsaro D, Mrva M, Colson P & Walochnik J (2024) Validation and redescription of Acanthamoeba terricola Pussard, 1964 (Amoebozoa: Acanthamoebidae) European Journal of Protistology 94: 126091.
Division/Phylum: Amoebozoa Class: Discosea
Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology
and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee
that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive
from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and
Adl et al. (2019) for protists.
Culture media, purity and growth conditions:
Medium:
NN;
Monoxenic; maintained by serial subculture and cryopreserved; non-pathogenic E. coli food source
| Attributes | |
| Authority | (Douglas) Volkonsky 1931 |
| Isolator | Neff (1957) |
| Collection Site | Pacific Grove, California |
| Notes | Isolated via plating and migration; Derived from the same Neff isolate as CCAP 1501/1A; This strain may be Acanthamoeba terricola Pussard 1964, according to Corsaro et al. 2024, our sequence (although short) is a 100% match to both species, for now we are retaining the original strain name (Nov24) |
| Axenicity Status | Monoxenic |
| Area | North America |
| Country | USA |
| Environment | Soil |
| GMO | No |
| Group | Protozoa |
| In Scope of Nagoya Protocol | No |
| ABS Note | Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain. |
| Collection Date | 1957 |
| Pathogen | Potential Pathogen: Hazard Class 2 |
| Strain Maintenance Sheet | SM_Acanthamoeba.pdf |
| Toxin Producer | Not Toxic / No Data |
| Type Culture | No |
| Taxonomy WoRMS ID | |
CCAP 1501/1B
Acanthamoeba castellanii "Neff Strain"
- Product Code: CCAP 1501/1B
- Availability: Potential Pathogen
You are ordering Pathogenic strains - we cannot supply these via online ordering. Please contact CCAP directly. You will be required to download and submit Form A before any pathogenic strains are despatched.