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References [ 10 ]
Costas M & Griffiths AJ (1984) The esterases and acid-phosphatases of Acanthamoeba (Amoebida, Acanthamoebidae). Protistologica 20(1): 33-41.
DOI: none
Daggett PM, Sawyer T & Nerad T (1982) Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microbial Ecology 8(4): 371-386.
Nakisah MA, Fatimah H, Manaf AM & Aspollah MS (2008) Observation on the cytotoxicity of a plant compound labeled as MK2 on morphology of Acanthamoeba by Scanning Electron Microscopy. Annals of Microscopy 8: 73-76.
DOI: none
Nakisah MA, Faezah SS & Mohd TN (2014) Discovery of proteins in Acanthamoeba polyphaga with potential as biomarker for ammonium chloride contamination. Asian pacific Journal of Tropical Disease 4: 252.
Inglis TJ, Rigby P, Robertson TA, Dutton NS, Henderson M & Chang BJ (2000) Interaction between Burkholderia pseudomallei and Acanthamoeba species results in coiling phagocytosis, endamebic bacterial survival, and escape. Infection and Immunity 68: 1681-1686.
Marciano-Cabral F & Cabral G (2003) Acanthamoeba spp. as agents of disease in humans. Clinical Microbiological Reviews 16: 273-307.
De Jonckheere JF (1980) Growth characteristics, cytopathic effect in cell culture, and virulence in mice of 36 type strains belonging to 19 different Acanthamoeba spp. Applied and Environmental Microbiology 39: 681-685.
DOI: none
Hsueh TY & Gibson KE (2015) Transfer of Acanthamoeba spp. to fresh produce from water and environmental surfaces. Letters in Applied Microbiology 61: 192-198.
Fuerst, PA (2023) The status of molecular analyses of isolates of Acanthamoeba maintained by international culture collections. Microorganisms 11(2): 295.
Fuerst PA & Booton GC (2020) Species, sequence types and alleles: Dissecting genetic variation in Acanthamoeba Pathogens 9(7): 534.
Sequences [ 1 ]
EMBL/Genbank Links
(Bold text = submission by CCAP staff or collaborators)
Division/Phylum: Amoebozoa Class: Discosea

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: NN; Monoxenic; maintained by serial subculture and cryopreserved; non-pathogenic E. coli food source.
Attributes
Authority(Puschkarew) Volkonsky 1931
IsolatorPage (1964)
Collection Sitemill pond Madison, Wisconsin, USA
Notes Clonal culture; cystformer; isolated via plating
Axenicity Status Monoxenic
Area North America
Country USA
Environment Freshwater
GMO No
Group Protozoa
In Scope of Nagoya Protocol No
ABS Note Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain.
Collection Date 1964
Original Designation 23
Pathogen Potential Pathogen: Hazard Class 2
Strain Maintenance Sheet SM_Acanthamoeba.pdf
Toxin Producer Not Toxic / No Data
Type Culture No
Taxonomy WoRMS ID 163677
Equivalent StrainsATCC 30871

CCAP 1501/3A

Acanthamoeba polyphaga

  • Product Code: CCAP 1501/3A
  • Availability: Potential Pathogen
You are ordering Pathogenic strains - we cannot supply these via online ordering. Please contact CCAP directly. You will be required to download and submit Form A before any pathogenic strains are despatched.