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References [ 8 ]
Costas M & Griffiths AJ (1984) The esterases and acid-phosphatases of Acanthamoeba (Amoebida, Acanthamoebidae). Protistologica 20(1): 33-41.
DOI: none
Cervero-Arago S, Rodriguez-Martinez S, Canals O, Salvado H & Araujo RM (2014) Effect of thermal treatment on free-living amoeba inactivation. Journal of Applied Microbiology 116: 728-736.
Cervero-Aragó S, Sommer R & Araujo RM (2014) Effect of UV irradiation (253.7 nm) on free Legionella and Legionella associated with its amoebae hosts. Water Research 67: 299-309.
Cervero-Aragó S, Rodríguez-Martínez S, Puertas-Bennasar A & Araujo M (2015) Effect of common drinking water disinfectants, chlorine and heat, on free Legionella and amoebae-associated Legionella. PLoS ONE 10(8): e0134726.
Gonzales-Gustavson E, Cárdenas-Youngs Y, Calvo M, Figueira Marques da Silva M, Hundesa A, Amarós I, Moreno Y, Moreno-Mesonero L, Rosell R, Ganges L, Araujo R & Girones R (2017) Characterization of the efficiency and uncertainty of skimmed milk flocculation for the simultaneous concentration and quantification of water-borne viruses, bacteria and protozoa Journal of Microbiological Methods 134: 46-53.
Van Kenhove E, De Backer L, Janssens A & Laverge J (2019) Simulation of Legionella concentration in domestic hot water: comparison of pipe and boiler models Journal of Building Performance Simulation 12: 595-619.
Gabriel AA & Panaligan DC (2020) Heat and chlorine resistance of a soil Acanthamoeba sp. cysts in water Journal of Applied Microbiology -: -.
Fuerst, PA (2023) The status of molecular analyses of isolates of Acanthamoeba maintained by international culture collections. Microorganisms 11(2): 295.
Division/Phylum: Amoebozoa Class: Discosea

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: NN; Monoxenic; maintained by serial subculture and cryopreserved; non-pathogenic E. coli food source.
Attributes
Authority(Douglas) Volkonsky 1931
IsolatorLewin (1951)
Collection Sitegreenhouse tank Yale
Axenicity Status Monoxenic
Area North America
Country USA
Environment Freshwater
GMO No
Group Protozoa
In Scope of Nagoya Protocol No
ABS Note Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain.
Collection Date c 1951
Pathogen Potential Pathogen: Hazard Class 2
Strain Maintenance Sheet SM_Acanthamoeba.pdf
Toxin Producer Not Toxic / No Data
Type Culture No
Taxonomy WoRMS ID
Synonyms Hartmannella castellanii Douglas
Formerly Listed in CCAP asHartmannella castellanii

CCAP 1534/2

Acanthamoeba castellanii

  • Product Code: CCAP 1534/2
  • Availability: Potential Pathogen
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