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References [ 2 ]
Spanner C, Darienko T, Biehler T, Sonntag B & Pröschold T (2020) Endosymbiotic green algae in Paramecium bursaria: A new isolation method and a simple diagnostic PCR approach for the identification Diversity 12: 240.
Spanner C, Darienko T, Filker S, Sonntag B & Pröschold T (2022) Morphological diversity and molecular phylogeny of five Paramecium bursaria (Alveolata, Ciliophora, Oligohymenophorea) syngens and the identification of their green algal endosymbionts. Scientific Reports 12: 18089.
Sequences [ 1 ]
EMBL/Genbank Links
(Bold text = submission by CCAP staff or collaborators)
SSU (18S) + ITS1+2
Division/Phylum: Ciliates/Ciliophora Class: Oligohymenophorea Order: Peniculida

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: SES; Bacteria and other organisms present; maintained by serial subculture; contains green algae
Attributes
AuthoritySpanner, Darienko, Filker, Sonntag & Pröschold 2022
IsolatorUnknown
Collection Sitepond Burlington, North Carolina
Notes Green algal endosymbiont: Chlorella variabilis (NC64A = CCAP 211/84); Syngen R4; renamed after Spanner et al. 2022 (see References)
Axenicity Status Bacteria and other organisms present
Area North America
Country USA
Environment Freshwater
GMO No
Group Protozoa
In Scope of Nagoya Protocol No
Collection Date before 1990
Original Designation CIL-16
Pathogen Not pathogenic: Hazard Class 1
Strain Maintenance Sheet SM_Parameciumbursaria.pdf
Toxin Producer Not Toxic / No Data
Type Culture No
Taxonomy WoRMS ID
Equivalent StrainsCCAP 1660/40 (clone)
Other DesignationsCBS-13-1548
Formerly Listed in CCAP asParamecium bursaria

CCAP 1660/25

Paramecium tetratobursaria