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References [ 2 ]
Farag, A (2022) An integrated approach for landfill leachate treatment using Plasma/UV pre-treatment and indigenous green microalgae PhD Thesis, University of Sheffield -: -.
DOI: none
Farag AT, Holmes TD, Gilmour DJ & Zimmerman WB (2023) An integrated novel plasma-microalgae approach for landfill leachate treatment using a high-ammonia tolerant strain of Chlorella vulgaris Algal Research -: -.
Sequences [ 1 ]
EMBL/Genbank Links
(Bold text = submission by CCAP staff or collaborators)
SSU (18S)
Division/Phylum: Chlorophyta Class: Trebouxiophyceae Order: Chlorellales

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: 3N-BBM+V; Bacteria present; maintained by serial subculture and cryopreserved; 20 deg C
Attributes
AuthorityBeijerinck 1890
IsolatorFarag (2018)
Collection SiteErin landfill leachate treatent site (owned by Viridor) Chesterfield, UK
Climatic Zone Temperate
Notes from soil samples with leachate runoff from a landfill leachate treatment site; isolated via streaking cells across agar plates for several subcultures; identified by morphology & sequencing; This is a natural mutant of a Chlorella vulgaris strain collected and tested in different diluted concentrations of landfill leachate. This mutant strain is a high ammonia-N tolerant strain that is capable of growth and ammonia-N uptake in 20% water-diluted landfill leachate (v/v) with 75% ammonia-N removal (compared to its peers).
Axenicity Status Bacteria present
Area Europe
Country UK
Environment Soil
GMO No
Latitude 53 14' 42.6" N
Longitude 1 19' 45.9" W
In Scope of Nagoya Protocol No
ABS Note Collected post Nagoya Protocol from a country with no ABS measures at time of access. No known Nagoya Protocol restrictions for this strain.
Collection Date 05/2018
Original Designation ATFGM*
Pathogen Not pathogenic: Hazard Class 1
Strain Maintenance Sheet SM_FreshwaterGreens.pdf
Toxin Producer Not Toxic / No Data
Type Culture No
Taxonomy WoRMS ID 532029

CCAP 211/141

Chlorella vulgaris


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