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References [ 76 ]
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Hochmuth JD, Asselman J & De Schamphelaere KAC (2014) Are interactive effects of harmful algal blooms and copper pollution a concern for water quality management? Water Research 60: 41-53.
Kolar B, Arnus L, Jeretin B, Gutmaher A, Drobne D & Durjava MK (2014) The toxic effect of oxytetracycline and trimethoprim in the aquatic environment. Chemosphere 115: 75-80.
Machado MD & Soares EV (2014) Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to metal stress. Aquatic Toxicology 147: 1-6.
Sjollema SB, van Beusekom SAM, van der Geest HG, Booij P, de Zwart D, Vethaak AD & Admiraal W (2014) Laboratory algal bioassays using PAM fluorometry: Effects of test conditions on the determination of herbicide and field sample toxicity. Environmental Toxicology and Chemistry 33: 1017-1022.
Goncalves AL, Simoes M & Pires JCM (2014) The effect of light supply on microalgal growth, CO2 uptake and nutrient removal from wastewater. Energy Conversion and Management 85: 530-536.
Verheyen L, Versieren L & Smolders E (2014) Natural dissolved organic matter mobilizes Cd but does not affect the Cd uptake by the green algae Pseudokirchneriella subcapitata (Korschikov) in resin buffered solutions. Aquatic Toxicology 154: 80-86.
Baken S, Nawara S, Van Moorleghem C & Smolders E (2014) Iron colloids reduce the bioavailability of phosphorous to the green alga Raphidocelis subcapitata. Water Research 59: 198-206.
De Schamphelaere KAC, Nys C & Janssen CR (2014) Toxicity of lead (Pb) to freshwater green algae: Development and validation of a bioavailability model and inter-species sensitivity comparison. Aquatic Toxicology 155: 348-359.
Van Doorslaer X, Haylamicheal ID, Dewulf J, Van Langenhove H, Janssen CR & Demeestere K (2014) Heterogeneous photocatalysis of moxifloxacin in water: Chemical transformation and ecotoxicity. Chemosphere 119: S75-S80.
Mater N, Geret F, Castillo L, Faucet-Marquis V, Albasi C & Pfohl-Leszkowicz A (2014) In vitro tests aiding ecological risk assessment of ciprofloxacin, tamoxifen and cyclophosphamide in range of concentrations released in hospital wastewater and surface water. Environment International 63: 191-200.
Magdaleno A, Saenz ME, Juárez AB & Moretton J (2015) Effects of six antibiotics and their binary mixtures on growth of Pseudokirchneriella subcapitata. Ecotoxicology and Environmental Safety 113: 72-78.
Machado MD, Lopes AR & Soares EV (2015) Responses of the alga Pseudokirchneriella subcapitata to long-term exposure to metal stress. Journal of Hazardous Materials 296: 82-92.
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Goncalves AL, Pires JCM & Simoes M (2016) Biotechnological potential of Synechocystis salina co-cultures with selected microalgae and cyanobacteria: Nutrients removal, biomass and lipid production. Bioresource Technology 200: 279-286.
Nys C, Janssen CR, Van Sprang P & De Schamphelaere KAC (2015) The effect of pH on chronic aquatic Ni toxicity is dependent on the pH itself: extending the chronic Ni bioavailability models Environmental Toxicology and Chemistry 35: 1097-1106.
Hochmuth JD, Janssen CR & De Schamphelaere KAC (2015) Temperature and food concentration have limited influence on the mixture toxicity of copper and Microcystis aeruginosa to Daphnia magna. Environmental Toxicology and Chemistry 35: 742-749.
Mouchet F, Gancet C, Flahaut E, Pinelli E, Boutonnet J & Gauthier L (2016) International standardized procedures for in vivo evaluation of multi-walled carbon nanotube toxicity in water. Toxicological and Environmental Chemistry 98: 829-847.
Goncalves AL, Rodrigues CM, Pires JCM & Simoes M (2016) The effect of increasing CO2 concentrations on its capture, biomass production and wastewater remediation by microalgae and cyanobacteria. Algal Research 14: 127-136.
Cerrillo C, Barandika G, Igartua A, Areitioaurtena O & Mendoza G (2016) Towards the standardization of nanoecotoxicity testing: Natural organic matter 'camouflages' the adverse effects of TiO2 and CeO2 nanoparticles on green microalgae. Science of the Total Environment 543: 95-104.
Gao C, De Schamphelaere KAC & Smolders E (2016) Zinc toxicity to the alga Pseudokirchneriella subcapitata decreases under phosphate limiting growth conditions. Aquatic Toxicology 173: 74-82.
Guo J, Selby K & Boxall A (2016) Comparing the sensitivity of chlorophytes, cyanobacteria and diatoms to major-use antibiotics. Environmental Toxicology and Chemistry 35: 2587-2596.
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Nys C, Van Regenmortel T, Janssen CR, Blust R, Smolders E & De Schamphelaere KAC (2016) Comparison of chronic mixture toxicity of Ni-Zn-Cu and Ni-Zn-Cu-Cd mixtures between Ceriodaphnia dubia and Pseudokirchneriella subcapitata. Environmental Toxicology and Chemistry 36: 1056-1066.
Yamagishi T, Yamaguchi H, Suzuki S, Horie Y & Tatarazako N (2017) Cell reproductive patterns in the green alga Pseudokirchneriella subcapitata (=Selenastrum capricornutum) and their variations under exposure to the typical toxicants potassium dichromate and 3,5-DCP PLoS ONE 12: e0171259.
Yamagishi T, Horie Y & Tatarazako N (2017) Synergism between macrohalide antibiotics and the azole fungicide ketoconazole in growth inhibition testing of the green alga Pseudokirchneriella subcapitata. Chemosphere 174: 1-7.
Krienitz L, Bock C, Nozaki H & Wolf M (2011) SSU rRNA gene phylogeny of morphospecies affiliated to the bioassay alga "Selenastrum capricornutum" recovered the polyphyletic origin of crescent-shaped chlorophyta. Journal of Phycology 47: 880-893.
Van Regenmortel T, Berteloot O, Janssen CR & De Schamphelaere KAC (2017) Analyzing the capacity of the Daphnia magna and Pseudokirchneriella subcapitata bioavailability models to predict chronic zinc toxicity at high pH and low Ca concentrations and formulation of a generalized bioavailability model (gBAM) for D. magna. Environmental Toxicology and Chemistry -: -.
Kim JW, Rehmann L & Ray MB (2017) Development of microalgal bioassay based on the community level physiological profiling (CLPP). Algal Research 25: 47-53.
Joo JH, Wang P, Park BS, Byun JH, Choi HJ, Kim SH & Han MS (2017) Improvement of cyanobacterial-killing biologically derived substances (BDSs) using an ecologically safe and cost-effective naphthoquinone derivative. Ecotoxicology and Environmental Safety 141: 188-198.
Van Regenmortel T & De Schamphelaere KAC (2018) Mixtures of Cu, Ni, and Zn act mostly noninteractively on Pseudokirchneriella subcapitata growth in natural waters. Environmental Toxicology and Chemistry 37: 587-598.
Mennillo E, Arukwe A, Monni G, Meucci V, Intorre L & Pretti C (2018) Ecotoxicological Properties of Ketoprofen and the S(+)-Enantiomer (Dexketoprofen): Bioassays in Freshwater Model Species and Biomarkers in Fish PLHC-1 Cell Line Environmental Toxicology and Chemistry 37: 201-212.
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Sequences [ 1 ]
EMBL/Genbank Links
(Bold text = submission by CCAP staff or collaborators)
Division/Phylum: Chlorophyta Class: Chlorophyceae Order: Sphaeropleales

Note: for strains where we have DNA barcodes we can be reasonably confident of identity, however for those not yet sequenced we rely on morphology and the original identification, usually made by the depositor. Although CCAP makes every effort to ensure the correct taxonomic identity of strains, we cannot guarantee that a strain is correctly identified at the species, genus or class levels. On this basis users are responsible for confirming the identity of the strain(s) they receive from us on arrival before starting experiments.
For strain taxonomy we generally use AlgaeBase for algae and Adl et al. (2019) for protists.

Culture media, purity and growth conditions:
Medium: EG:JM; Axenic; maintained by serial subculture and cryopreserved; liquid
Attributes
Authority(Korshikov) Nygaard, Komárek, Kristiansen & Skulberg 1987
IsolatorSkulberg (1959)
Collection Site River Nitelva, Akershus, Norway
Notes Images 1-5 by Tatyana Darienko. April 2017: renamed after Krienitz et al. 2011 (DOI: 10.1111/j.1529-8817.2011.01010.x), see also AlgaeBase (http://www.algaebase.org/search/species/detail/?species_id=cb2ab3ab941b845fc).
Axenicity Status Axenic
Area Europe
Country Norway
Environment Freshwater
GMO No
Latitude 59 58 N
Longitude 11 2 E
In Scope of Nagoya Protocol No
ABS Note Collected pre Nagoya Protocol. No known Nagoya Protocol restrictions for this strain.
Collection Date c 1959
Original Designation NIVA CHL 1
Pathogen Not pathogenic: Hazard Class 1
Special Uses recommended in ecotoxicity testing guidelines OECD 201 and 87/302/EEC
Strain Maintenance Sheet SM_FreshwaterEcotox.pdf
Toxin Producer Not Toxic / No Data
Type Culture No
Taxonomy WoRMS ID 610914
Equivalent Strains(?)SAG 61-81 as Ankistrodesmus bibraianus,ATCC 22662,UTCC 37,UTEX 1648
Synonyms Ankistrodesmus subcapitatus Korshikov 1953 , Kirchneria subcapitata (Korshikov) Hindák 1988 , Kirchneriella subcapitata Korshikov 1953 , Pseudokirchneriella subcapitata (Korshikov) Hindák 1990
Formerly Listed in CCAP asSelenastrum capricornutum Printz 1914

CCAP 278/4

Raphidocelis subcapitata


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